Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization.
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Spencer, Hamish G.
Taylor, Helen R.
Lamare, Miles D.
Gemmell, Neil J.
MetadataShow full item record
DNA extraction from environmental samples (environmental DNA; eDNA) for metabarcoding‐based biodiversity studies is gaining popularity as a noninvasive, time‐efficient, and cost‐effective monitoring tool. The potential benefits are promising for marine conservation, as the marine biome is frequently under‐surveyed due to its inaccessibility and the consequent high costs involved. With increasing numbers of eDNA‐related publications have come a wide array of capture and extraction methods. Without visual species confirmation, inconsistent use of laboratory protocols hinders comparability between studies because the efficiency of target DNA isolation may vary. We determined an optimal protocol (capture and extraction) for marine eDNA research based on total DNA yield measurements by comparing commonly employed methods of seawater filtering and DNA isolation. We compared metabarcoding results of both targeted (small taxonomic group with species‐level assignment) and universal (broad .....
JournalEcology and Evolution
Sustainable Development Goals (SDG)14.A
Best Practice TypeManual (incl. handbook, guide, cookbook etc)
CitationJeunen, G‐J.; Knapp, M.; Spencer, H.G.; et al. (2019) Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization. Ecology and Evolution, 9, pp.1323-1335. DOI:. https://doi.org/10.1002/ece3.4843
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